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Council Directive of 26 June 1990 on animal health conditions governing the movement and import from third countries of equidae (90/426/EEC) (repealed)

EU Directive 1990/426 · Adopted 26 June 1990 · Extends to United Kingdom

Original text. legislation.gov.uk does not revise this item, so later amendments are not shown here. Changes made to it are listed under each provision when known.

Council Directive

of 26 June 1990

on animal health conditions governing the movement and import from third countries of equidae

(90/426/EEC) (repealed)

F1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Annotations to the whole text

Textual amendments

F1

Repealed by Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance).

Contents

  1. Chapter 1: General provisions Repealed
  2. 1Article 1 Repealed
  3. 2Article 2 Repealed
  4. Chapter II: Rules for the movement of equidae Repealed
  5. 3Article 3 Repealed
  6. 4Article 4 Repealed
  7. 5Article 5 Repealed
  8. 6Article 6 Repealed
  9. 7Article 7 Repealed
  10. 8Article 8 Repealed
  11. 9Article 9 Repealed
  12. 10Article 10 Repealed
  13. Chapter III: Rules for imports from third countries Repealed
  14. 11Article 11 Repealed
  15. 12Article 12 Repealed
  16. 13Article 13 Repealed
  17. 14Article 14 Repealed
  18. 15Article 15 Repealed
  19. 16Article 16 Repealed
  20. 17Article 17 Repealed
  21. 18Article 18 Repealed
  22. 19Article 19 Repealed
  23. 20Article 20 Repealed
  24. 21Article 21 Repealed
  25. Chapter IV: Final provisions Repealed
  26. 22Article 22 Repealed
  27. 23Article 23 Repealed
  28. 24Article 24 Repealed
  29. 25Article 25 Repealed
  30. 26Article 26 Repealed
  31. 27Article 27 Repealed
  32. 28Article 28 Repealed
  33. Annex ACOMPULSORILY NOTIFIABLE DISEASES Repealed
  34. Annex BAnnex B Repealed
  35. Annex CAnnex C Repealed
  36. Annex DAFRICAN HORSE SICKNESS Repealed
  37. 1COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST) Repealed
  38. Test procedure Repealed
  39. Preparation of plates Repealed
  40. 1.1.1.1Division 1.1.1.1 Repealed
  41. 1.1.1.2Division 1.1.1.2 Repealed
  42. Control wells Repealed
  43. 1.1.2.1Division 1.1.2.1 Repealed
  44. 1.1.2.2Division 1.1.2.2 Repealed
  45. 1.1.2.3Division 1.1.2.3 Repealed
  46. 1.1.2.4Division 1.1.2.4 Repealed
  47. 1.1.3Spot test method Repealed
  48. 1.1.3.1Division 1.1.3.1 Repealed
  49. 1.1.4Serum titration method Repealed
  50. 1.1.4.1Division 1.1.4.1 Repealed
  51. Add 50 μl of guinea pig antisera, pre-diluted in blocking buffer, to all wells except the blank wells of the ELISA plate (all wells now contain a final volume of 100 μl). Repealed
  52. 1.1.5.1Division 1.1.5.1 Repealed
  53. 1.1.5.2Division 1.1.5.2 Repealed
  54. 1.1.5.3Division 1.1.5.3 Repealed
  55. 1.1.5.4Division 1.1.5.4 Repealed
  56. 1.1.5.5Division 1.1.5.5 Repealed
  57. 1.1.6Chromogen Repealed
  58. 1.1.7Reading Repealed
  59. Expression of results Repealed
  60. Repealed
  61. 1.2.1Using a software package print out the optical density (OD) values, and the percentage inhibition (PI) for test and control sera based on the mean value recorded in the four guinea pig control wells. The data expressed as OD and PI values are used to determine whether the test has performed within acceptable limits. The upper control limits (UCL) and lower control limits (LCL) for the guinea pig control are between OD values 1,4 and 0,4 respectively. The end-point titre for the positive control based on 50 % PI should be 1 in 240 (within a range from 1 in 120 to 1 in 480). Any plate that fails to conform to the above criteria must be rejected. However, if the positive control serum titre is greater than 1 in 480 and the test samples are still negative then the negative test samples can be accepted. Repealed
  62. 1.2.2The diagnostic threshold (cut-off value) for test sera is 50 % (PI 50 %). Samples recording PI values greater than 50 % are recorded as positive. Samples recording PI values lower than 50 % are recorded as negative. Repealed
  63. 2INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST) Repealed
  64. Test procedure Repealed
  65. Solid phase Repealed
  66. 2.1.1.1Division 2.1.1.1 Repealed
  67. 2.1.1.2Division 2.1.1.2 Repealed
  68. 2.1.1.3Division 2.1.1.3 Repealed
  69. 2.1.1.4Division 2.1.1.4 Repealed
  70. Test samples Repealed
  71. 2.1.2.1Serum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 μl per well. Incubate for 1 hour at 37 °C. Repealed
  72. 2.1.2.2Division 2.1.2.2 Repealed
  73. Conjugate Repealed
  74. 2.1.3.1Division 2.1.3.1 Repealed
  75. 2.1.3.2Division 2.1.3.2 Repealed
  76. Cromogen/Substrate Repealed
  77. 2.1.4.1Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6 mM DMAB (dimethyl aminobenzaldehyde) + 10 ml of 1,56 mM MBTH (3-methyl-2-benzo-thiazoline hydrazone hydrochlorid) + 5 μl H2O2) Repealed
  78. 2.1.4.2Division 2.1.4.2 Repealed
  79. Interpretation of the results Repealed
  80. Repealed
  81. 2.2.1Division 2.2.1 Repealed
  82. 2.2.2Division 2.2.2 Repealed
  83. 2.2.3Division 2.2.3 Repealed
  84. 2.2.4Division 2.2.4 Repealed
  85. 3BLOCKING ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST) Repealed
  86. Test procedure Repealed
  87. ELISA plates Repealed
  88. 3.1.1.1Division 3.1.1.1 Repealed
  89. 3.1.1.2Division 3.1.1.2 Repealed
  90. 3.1.1.3Division 3.1.1.3 Repealed
  91. Test samples and controls Repealed
  92. 3.1.2.1Division 3.1.2.1 Repealed
  93. 3.1.2.2Division 3.1.2.2 Repealed
  94. 3.1.3Conjugate Repealed
  95. 3.1.4Division 3.1.4 Repealed
  96. 3.1.5Chromogen/Substrate Repealed
  97. 3.1.6Reading Repealed
  98. Interpretation of the results Repealed
  99. 3.2.1Assay validation Repealed
  100. 3.2.2Cut-off calculation Repealed
  101. 3.2.3Interpretation of results Repealed