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Appendix 5 Determination of contamination level in IF and FISH testsF1

United Kingdom

Original text. Later amendments are not shown.

  1. 1..

    Count the mean number of typical fluorescent cells per field of view (c).

  2. 2..

    Calculate the number of typical fluorescent cells per microscope slide window (C).

    C = c × S/s

    where S = surface area of window of multispot slide
    and s = surface area of objective field
    s = πi 2 /4G 2 K 2 where i = field coefficient (varies from 8 to 24 depending upon ocular type)
    K = tube coefficient (1 or 1,25)
    G = magnification of objective (100 ×, 40 × etc.).
  3. 3..

    Calculate the number of typical fluorescent cells per ml of re-suspended pellet (N).

    N = C × 1 000 /y × F

    where y = volume of re-suspended pellet on each window
    and F = dilution factor of re-suspended pellet.

Annotations

Textual amendments

F1

Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..