Division CONTENTSF1

United Kingdom


	General principles
SECTION I:	Application of the test scheme
	1.	Detection scheme for the diagnosis of brown rot and bacterial wilt ( R. solanacearum ) in potato tubers and potato, tomato or other host plants with symptoms of brown rot or bacterial wilt
	2.	Scheme for detection and identification of R. solanacearum in samples of asymptomatic potato tubers
	3.	Scheme for detection and identification of R. solanacearum in samples of asymptomatic, potato, tomato or other host plants
SECTION II:	Detailed methods for detection of R. solanacearum in potato tubers and potato, tomato or other host plants with symptoms of brown rot or bacterial wilt
	1.	Symptoms
	2.	Rapid screening tests
	3.	Isolation procedure
	4.	Identification tests for R. solanacearum
SECTION III:	1.	Detailed methods for detection and identification of R. solanacearum in samples of asymptomatic potato tubers
		1.1.	Sample preparation
		1.2.	Testing
	2.	Detailed methods for detection and identification of R. solanacearum in samples of asymptomatic potato, tomato or other plants
		2.1.	Sample preparation
		2.2.	Testing
SECTION IV:	1.	Scheme for detection and identification of R. solanacearum in water
	2.	Methods for detection and identification of R. solanacearum in water
		2.1.	Sample preparation
		2.2.	Testing
SECTION V:	1.	Scheme for detection and identification of R. solanacearum in soil
	2.	Methods for detection and identification of R. solanacearum in soil
		2.1.	Samples preparation
		2.2.	Testing
SECTION VI:	Optimised protocols for detection and identification of R. solanacearum
	A	Diagnostic and detection tests
		1.	Stem streaming test
		2.	Detection of poly-ß-hydroxybutyrate granules
		3.	Serological agglutination tests
		4.	Selective isolation
			4.1.	Selective plating
			4.2.	Enrichment procedure
		5.	Immunofluorescence test (IF test)
		6.	Polymerase chain reaction test (PCR test)
			6.1.	DNA purification methods
				(a)	Method according to Pastrik (2000)
				(b)	Other methods
			6.2.	PCR
			6.3.	Analysis of the PCR product
		7.	Fluorescent in-situ hybridization test (FISH test)
		8.	Enzyme Linked ImmunoSorbent Assay (ELISA) tests
			(a)	Indirect ELISA
			(b)	DASI (Double-Antibody Sandwich Indirect) ELISA
		9.	Bioassay test
	B	Identification tests
		1.	Nutritional and enzymatic identification tests
		2.	IF test
		3.	ELISA test
		4.	PCR test
		5.	FISH test
		6.	Fatty acid profiling (FAP)
		7.	Strain characterisation methods
			7.1.	Biovar determination
			7.2.	Genomic fingerprinting
			7.3.	PCR methods
	C	Confirmation test
		Appendix 1		Laboratories involved in optimisation and validation of protocols
		Appendix 2		Media for isolation and culture of R. solanacearum
		Appendix 3		(A)	Commercially available standardised control material
				(B)	Preparation of controls
		Appendix 4		Buffers for test procedures
		Appendix 5		Determination of contamination level in IF and FISH tests
		Appendix 6		Validated PCR protocols and reagents
		Appendix 7		Validated reagents for FISH test
		Appendix 8		Culture conditions for tomato and eggplant
		References

Annotations

Textual amendments

F1: Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..