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  1. 1..

    F1Prepare a smear of bacterial ooze from infected tissue or from a 48-hour culture on YPGA or SPA medium (Appendix 2) on a microscope slide.

  2. 2..

    Prepare positive control smears of a biovar 2 strain of R. solanacearum and, if considered useful, a negative control smear of a known PHB negative sp.

  3. 3..

    Allow to air dry and pass the lower surface of each slide rapidly above a flame to fix the smears.

  4. 4..

    Stain preparation with either Nile Blue or Sudan Black and observe microscopically as described below:

Annotations

Textual amendments

F1

Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..