OpenLegislation

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United Kingdom

Original text. Later amendments are not shown.

  1. (i)

    F1According to test slide preparation in 5.1(i):

    Prepare a set of twofold dilutions The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).

  2. (ii)

    According to test slide preparation in 5.1(ii):

    Prepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity.

Figure 1.

Preparation of the test slide according to 5.1(i) and 5.3(i)

Dilutions of resuspended pellet
1/100 1/1 1/1 1/1 1/1 Dilution of resuspended pellet
(T = titre) T/2 T/4 T/2 T 2T Twofold dilutions of antiserum/antibody
Sample 1
1 2 3 4 5
Duplicate of sample1 or sample 2
6 7 8 9 10

Figure 2.

Preparation of the test slide according to 5.1(ii) and 5.3(ii).

Working dilution of antiserum/antibody
1/1 1/10 1/100 empty empty Decimal dilution of resuspended pellet
Sample 1
1 2 3 4 5
Duplicate of sample 1 or sample 2
6 7 8 9 10

Annotations

Textual amendments

F1

Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..