Division 1.3 Criteria for microbiological analyses at source

These analyses shall include:

1. 1.3.1.

   a demonstration of the absence of parasites and pathogenic micro-organisms;

2. 1.3.2.

   a quantitative determination of the revivable colony count indicative of faecal contamination:

   1. (a)

      absence of Escherichia coli and other coliforms in 250 ml at 37 °C and 44,5 °C;

   2. (b)

      absence of faecal streptococci in 250 ml;

   3. (c)

      absence of sporulated sulphite-reducing anaerobes in 50 ml;

   4. (d)

      absence of Pseudomonas aeruginosa in 250 ml;

3. 1.3.3.

   determination of the revivable total colony count per ml of water:

   1. (a)

      at 20 to 22 °C in 72 hours on agar-agar or an agar-gelatine mixture;

   2. (b)

      at 37 °C in 24 hours on agar-agar.