Division 3.3 ProcedureF1
United Kingdom Repealed
- 3.3.1Oxidation of the L-ascorbic acid to dehydroascorbic acid
- 3.3.2Formation and extraction of the bis(2,4-dinitrophenylhydrazone) derivative of diketogulonic acid
- 3.3.3Separation of the bis(2,4-dinitrophenylhydrazone) by chromatography; this is to be carried out in the 2 hours following the extraction (3.3.2).
- 3.3.4Preparation of the calibration curve
- 3.3.5Expression of results