Division 4 REAGENTSF1
United Kingdom
- 4.1.
Potassium hydroxide minimum titre 85 %.
- 4.2.
Potassium hydroxide ethanolic solution, approximately 2 M.
Dissolve 130 g of potassium hydroxide (4.1) with cooling in 200 ml of distilled water and then make up to one litre with ethanol (4.7). Keep the solution in well-stoppered dark glass bottles and stored maximum 2 days.
- 4.3.
Ethyl ether, for analysis quality.
- 4.4.
Anhydrous sodium sulphate, for analysis quality.
- 4.5.
Acetone, for chromatography quality.
- 4.6.
Ethyl ether, for chromatography quality.
- 4.7.
Ethanol of analytical quality.
- 4.8.
Ethyl acetate of analytical quality.
- 4.9.
Internal standard, α-cholestanol, purity more than 99 % (purity must be checked by GC analysis).
- 4.10.
Internal standard solution of α-cholestanol, 0,2 solution (m/V) in ethyl acetate (4.8).
- 4.11.
Phenolphthalein solution, 10 g/L in ethanol (4.7).
- 4.12.
A 0,1 % (m/v) solution of 1-eicosanol in ethyl acetate (internal standard).