Division 3 APPARATUSF1

The usual laboratory equipment and in particular the following:

1. 3.1.

   Complete apparatus for analysis by thin-layer chromatography using 20 × 20 cm glass plates.

2. 3.2.

   Ultraviolet lamp with a wavelength of 366 or 254 nm.

3. 3.3.

   Microsyringes, 100 μL and 500 μL.

4. 3.4.

   Cylindrical filter funnel with a G3 porous septum (porosity 15-40 μm) of diameter approximately 2 cm and a depth of 5 cm, suitable for filtration under vacuum with male ground-glass joint.

5. 3.5.

   Conical flask with ground-glass female joint, 50 mL which can be fitted to the filter funnel (3.4).

6. 3.6.

   Test tube with a tapering bottom and a sealing glass stopper, 10 mL.

7. 3.7.

   Calcium dichloride desiccator.

8. 3.8.

   HPLC system, consisting of:

   1. 3.8.1.

      Binary pump.

   2. 3.8.2.

      Manual or automatic injector equipped with 200 μL injection loop.

   3. 3.8.3.

      In-line degasser.

   4. 3.8.4.

      UV-VIS or IR detector.

9. 3.9.

   HPLC column (25 cm × 4 mm i.d.) with silica gel 60 (5 μm particle size).

10. 3.10.

    Syringe filter, 0,45 μm.

11. 3.11.

    Conical flask 25 mL.