Division 4 REAGENTSF1
United Kingdom
- 4.1.
Potassium hydroxide minimum titre 85 %.
- 4.2.
Potassium hydroxide ethanolic solution, approximately 2 M.
Dissolve 130 g of potassium hydroxide (4.1) with cooling in 200 ml of distilled water and then make up to one litre with ethanol (4.9). Keep the solution in well-stoppered dark glass bottles and stored maximum 2 days.
- 4.3.
Ethyl ether, for analysis quality.
- 4.4.
Potassium hydroxide ethanolic solution, approximately 0,2 M.
Dissolve 13 g of potassium hydroxide (4.1) in 20 ml of distilled water and make up to one litre with ethanol (4.9).
- 4.5.
Glass 20x20 plates coated with silica gel, without fluorescence indicator, thickness 0,25 mm (commercially available ready for use).
- 4.6.
Acetone, for chromatography quality.
- 4.7.
n-Hexane, for chromatography quality.
- 4.8.
Ethyl ether, for chromatography quality.
- 4.9.
Ethanol of analytical quality.
- 4.10.
Ethyl acetate of analytical quality.
- 4.11.
Reference solution for thin-layer chromatography: cholesterol, phytosterols, alcohols and Erythrodiol 5 % solution in Ethyl acetate (4.10).
- 4.12.
Solution of 2,7-dichlorofluorescein, 0,2 % in ethanolic solution. Make slightly basic by adding a few drops of 2 M alcoholic potassium hydroxide solution (4.2).
- 4.13.
n-Hexane (4.7)/ethyl ether (4.8) mixture 65:35 (V/V).
- 4.14.
HPLC mobile phase n-hexane (4.7)/ethyl ether (4.8) (1:1) (V/V).