Division 5.5 Quantitative analysis of the waxesF1

United Kingdom

Determine the area of the peaks corresponding to the lauryl arachidate internal standard and the aliphatic esters from C ₄₀ to C ₄₆ with the aid of the integrator.

Determine the total waxes content by adding each individual wax, in mg/kg of fat, as follows:

$Waxes, mg ∕ kg = \frac{(\sum A_{x}) \times m_{s} \times 1000}{A_{s} \times m}$

where:

A _x

area corresponding to the peak for the individual ester, in computer counts

A _s

area corresponding to the peak for the lauryl arachidate internal standard, in computer counts

m _s

mass of the lauryl arachidate internal standard added, in milligrams;

mass of the sample taken for determination, in grams.

5.5.1Quantitative analysis of the methyl and ethyl esters

Annotations

Textual amendments

F1: Inserted by Commission Regulation (EU) No 61/2011 of 24 January 2011 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.

Notes on Annex XX

Footnotes

f1: After elution of the sterol esters, the chromatogram should not show any significant peaks (triacylglycerols).