Division 5.5.1 Quantitative analysis of the methyl and ethyl estersF1

United Kingdom

With the aid of the integrator, determine the areas of the peaks corresponding to the methyl heptadecanoate internal standard, the methyl esters of the C ₁₆ and C ₁₈ fatty acids and the ethyl esters of the C ₁₆ and C ₁₈ fatty acids.

Determine the content of each alkyl ester, in mg/kg of fat, as follows:

$Ester, mg ∕ kg = \frac{A_{x} \times m_{s.} \times 1000}{A_{s} \times m}$

where:

A _x

area corresponding to the peak for the individual C ₁₆ and C ₁₈ ester, in computer counts

A _s

area corresponding to the peak for the methyl heptadecanoate internal standard, in computer counts

m _s

mass of the methyl heptadecanoate internal standard added, in milligrams;

mass of the sample taken for determination, in grams.

Annotations

Textual amendments

F1: Inserted by Commission Regulation (EU) No 61/2011 of 24 January 2011 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.

Notes on Annex XX

Footnotes

f1: After elution of the sterol esters, the chromatogram should not show any significant peaks (triacylglycerols).