Annex II

United Kingdom

No (or EC No)	Additive	Chemical formula, decription	Species or category of animal	Maximum age	Minimum content	Maximum content	Other provisions	Period of authorisation
1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C.
1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 °C.
1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 4,8 and 50 °C.
1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 and 37 °C.
1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C.
1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.
No (or EC No)	Additive	Chemical formula, decription	Species or category of animal	Maximum age	Units of activity/kg complete feedingstuff	Units of activity/kg complete feedingstuff	Other provisions	Period of authorisation
23	Endo-1,4-beta-xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum of activity of: Solid form: 70 000 IFP ^f1/g Liquid form: 7 000 IFP/ml	Turkeys for fattening	—	700 IFP	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: 1 400 IFP 3. For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat	28.2.2005
23	Endo-1,4-beta-xylanase EC 3.2.1.8		Laying hens	—	840 IFP	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: 840 IFP 3. For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat	28.2.2005
27	Endo-1,4-beta-xylanase EC 3.2.1.8 Endo-1,3(4)-beta-glucanase EC 3.2.1.6	Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 529.94) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of: Solid form: 200 000 BXU ^f2/g 200 000 BU ^f3/g Liquid form: 30 000 BXU/g 30 000 BU/g	Piglets	2 months	7 500 BXU	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: 7 500 to 15 000 BXU 7 500 to 15 000 BU 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat	28.2.2005
27			Piglets	2 months	7 500 BU	—		28.2.2005
28	3-Phytase EC 3.1.3.8	Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum activity of Solid form: 5 000 PPU ^f4/g Liquid form: 1 000 PPU/g	Chickens for fattening	—	500 PPU	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: 500 to 750 PPU 3. For use in compound feed containing more than 0,22 % phytin bound phosphorus	28.2.2005
30	Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,-beta-xylanase EC 3.2.1.8	Preparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of: Powder form: Endo-1,3(4)-beta-glucanase: 2 000 U ^f5/g Endo-1,4-beta-xylanase: 1 400 U/g ^f6/g Liquid form: Endo-1,3(4)-beta-glucanase: 500 U/ml Endo-1,4-beta-xylanase: 350 U/ml	Turkeys for fattening	—	Endo-1,3(4)-beta-glucanase: 100 U	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: endo-1,3(4)-beta-glucanase: 100 U endo-1,4-beta-xylanase: 70 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat	28.2.2005
			Turkeys for fattening	—	Endo-1,4-beta-xylanase: 70 U	—		28.2.2005
			Laying hens	—	Endo-1,3(4)-beta-glucanase: 100 U	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stabiltiy to pelleting 2. Recommended dose per kilogram of complete feedingstuff: endo-1,3(4)-beta-glucanase: 100 U endo-1,4-beta-xylanase: 70 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta- glucans and arabinoxylans), e.g. continaing more than 60 % barley or 30 % wheat	28.2.2005
			Laying hens	—	Endo-1,4-beta-xylanase: 70 U	—		28.2.2005
			Pigs for fattening	—	Endo-1,3(4)-beta-glucanase: 100 U	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kilogram of complete feedingstuff: endo-1,3(4)-beta-glucanase: 100 U endo-1,4-beta-xylanase: 70 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % barley or 60 % wheat	28.2.2005
			Pigs for fattening	—	Endo-1,4-beta-xylanase: 70 U	—		28.2.2005
59	Endo-1,4-beta-xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: Endo-1,4-beta-xylanase: 300 U ^f7/g Endo-1,3(4)-beta-glucanase: 150 U ^f8/g Subtilisin: 4 000 U ^f9/g Alpha-amylase: 400 U ^f10/g Polygalacturonase: 25 U ^f11/g	Chickens for fattening	—	Endo-1,4-beta-xylanase: 300 U	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stbility to pelleting 2. Recommended dose per kilogram of complete feedingstuff: endo-1,4-beta-xylanase: 300 U endo-1,3(4)-beta-glucanase: 150 U subtilisin: 4 000 U alpha-amylase: 400 U polygalacturonase: 25 U 3. For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize	28.2.2005
	Endo-1,3(4)-beta-glucanase EC 3.2.1.6				Endo-1,3(4)-beta-glucanase: 150 U	—
	Subtilisin EC 3.4.21.62				Subtilisin: 4 000 U	—
	Alpha-amylase EC 3.2.1.1				Alpha-amylase: 400 U	—
	Polygalacturonase EC 3.2.1.15				Polygalacturonase: 25 U	—
60	Endo-1,4-beta-xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activitiy of: Endo-1,4-beta-xylanase: 5 000 U /g Endo-1,3(4)-beta-glucanase: 50 U /g	Chickens for fattening	—	Endo-1,4-beta-xylanase: 500 U	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 2. Recommended dose per kilogram of complete feedingstuff: endo-1,4-beta-xylanase: 500 to 2 500 U endo-1,3(4)-beta-glucanase: 5 to 25 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat	28.2.2005
60	Endo-1,3(4)-beta-glucanase EC 3.2.1.6		Chickens for fattening	—	Endo-1,3(4)-beta-glucanase: 5 U	—		28.2.2005
61	Endo-1,4-beta-xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of: Powder form: Endo-1,(4)-beta-xylanase: 17 000 BXU /g Endo-1,3(4)-beta-xylanase: 11 000 BU/ /g Liquid form: Endo-1,4-beta-xylanase: 22 000 U/ml Endo-1,3(4)-beta-glucanase: 15 000 U/ml	Chickens for fattening	—	Endo-1,4-beta-xylanase: 17 000 BXU	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting 2. Recommended dose per kilogram of complete feedingstuff: endo-1,4-beta-xylanase: 17 000 BXU endo-1,3(4)-beta-glucanase: 11 000 BU 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % barley or 55 % wheat	28.2.2005
61	Endo-1,3(4)-beta-glucanase EC 3.2.1.6		Chickens for fattening	—	Endo-1,3(4)-beta-glucanase: 11 000 BU	—		28.2.2005

Annotations

Footnotes

f1: 1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C.
f2: 1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 °C.
f3: 1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 4,8 and 50 °C.
f4: 1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 and 37 °C.
f5: 1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C.
f6: 1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C.
f7: 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.
f8: 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
f9: 1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
f10: 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.
f11: 1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.