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Annex IV

United Kingdom

Original text. Later amendments are not shown.

In the Annex to Regulation (EC) No 833/2005, the entry for E 1627 is replaced by the following:

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.’
‘E 1627

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Endo-1,4-beta-xylanase EC 3.2.1.8

Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

powder form:

  • endo-1,3(4)-beta-glucanase: 800 Uf1/g

  • endo-1,4-beta-xylanase: 800 Uf2/g

liquid form:

  • endo-1,3(4)-beta-glucanase: 800 U/ml

  • endo-1,4-beta-xylanase: 800 U/ml

Pigs for fattening—endo-1,3(4)-beta-glucanase: 400 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • endo-1,3(4)-beta-glucanase: 400 U

  • endo-1,4-beta-xylanase: 400 U.

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans) e.g. containing more than 65 % barley.

Without a time-limit
endo-1,4-beta-xylanase: 400 U—

Annotations

Footnotes

f1

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

f2

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.’