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Annex III

United Kingdom

Original text. Later amendments are not shown.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.

EC NoAdditiveChemical formula, descriptionSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1623

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Endo-1,4-beta-xylanase EC 3.2.1.8

Subtilisin EC 3.4.21.62

Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:

  • endo-1,3(4)-beta-glucanase: 200 Uf1/g

  • endo-1,4-beta-xylanase: 5 000 Uf2/g

  • subtilisin: 1 600 Uf3/g

Chickens for fattening—endo-1,3(4)-beta-glucanase: 25 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • endo-1,3(4)-beta-glucanase: 25-100 U

  • endo-1,4-beta-xylanase: 625-2 500 U

  • subtilisin: 200-800 U.

3.

For use in compound feed, e.g. containing more than 30 % wheat and 10 % barley.

Without a time-limit
endo-1,4-beta-xylanase: 625 U—
subtilisin: 200 U—

Annotations

Footnotes

f1

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

f2

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

f3

1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.