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Commission Regulation (EC) No 273/2008 of 5 March 2008 laying down detailed rules for the application of Council Regulation (EC) No 1255/1999 as regards methods for the analysis and quality evaluation of milk and milk products (repealed)

EU Regulation No 273/2008 · Adopted 5 March 2008 · Extends to United Kingdom

Original text. legislation.gov.uk does not revise this item, so later amendments are not shown here. Changes made to it are listed under each provision when known.

Commission Regulation (EC) No 273/2008

of 5 March 2008

laying down detailed rules for the application of Council Regulation (EC) No 1255/1999 as regards methods for the analysis and quality evaluation of milk and milk products (repealed)

F1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Annotations to the whole text

Textual amendments

F1

Repealed by Commission Implementing Regulation (EU) 2018/150 of 30 January 2018 amending Implementing Regulation (EU) 2016/1240 as regards methods for the analysis and quality evaluation of milk and milk products eligible for public intervention and aid for private storage.

Contents

  1. Chapter I: GENERAL PROVISIONS Repealed
  2. 1Subject matter and scope Repealed
  3. 2Routine methods Repealed
  4. Chapter II: METHODS OF ANALYSIS Repealed
  5. 3Evaluation of compliance of a consignment with a legal limit Repealed
  6. 4Sensory evaluation Repealed
  7. 5Tracers Repealed
  8. 6Detection of cows’ milk casein Repealed
  9. 7Detection of coliforms Repealed
  10. 8Determination of the lactose content Repealed
  11. 9Detection of rennet whey Repealed
  12. 10Detection of buttermilk Repealed
  13. 11Detection of antimicrobiotic residues Repealed
  14. 12Determination of skimmed-milk powder content Repealed
  15. 13Detection of starch Repealed
  16. 14Determination of moisture content in dried cream Repealed
  17. 15Determination of moisture content of acid buttermilk powder Repealed
  18. 16Determination of milk fat purity Repealed
  19. Chapter III: GENERAL AND FINAL PROVISIONS Repealed
  20. 17Quality assurance Repealed
  21. 18Sampling and disputes over the results of analysis Repealed
  22. 19Transition period Repealed
  23. 19aNotifications Repealed
  24. 20Repeals Repealed
  25. 21Entry into force Repealed
  26. Signature
  27. Annex I: (Article 1) Repealed
  28. LIST OF REFERENCE METHODS Repealed
  29. Annex II: (Article 3) Repealed
  30. EVALUATION OF COMPLIANCE OF A CONSIGNMENT WITH THE LEGAL LIMIT Repealed
  31. 1PRINCIPLE Repealed
  32. 2THE METHOD IS VALIDATED THROUGH INTERNATIONAL COLLABORATION Repealed
  33. 3IN-HOUSE VALIDATION WITH CALCULATION OF INTERNAL REPRODUCIBILITY STANDARD DEVIATION Repealed
  34. Annex III: (Article 4) Repealed
  35. EVALUATION OF ASSESSORS AND THE RELIABILITY OF RESULTS IN SENSORY ANALYSES Repealed
  36. Division Repealed
  37. ADETERMINATION OF THE ‘REPEATABILITY INDEX’ Repealed
  38. BDETERMINING THE ‘DEVIATION INDEX’ Repealed
  39. CCOMPARISON OF THE RESULTS OBTAINED IN DIFFERENT REGIONS OF A MEMBER STATE AND IN DIFFERENT MEMBER STATES Repealed
  40. Annex IV: (Article 4) Repealed
  41. SENSORY EVALUATION OF BUTTER Repealed
  42. 1SCOPE Repealed
  43. 2DEFINITIONS Repealed
  44. 3TEST ROOM Repealed
  45. SELECTION OF ASSESSORS Repealed
  46. Division Repealed
  47. 4.1Division 4.1 Repealed
  48. 4.2Division 4.2 Repealed
  49. 5REQUIREMENTS FOR THE PANEL Repealed
  50. 6PERFORMANCE Repealed
  51. 7SAMPLING AND PREPARATION OF THE SAMPLE Repealed
  52. ASSESSMENT OF THE VALUE OF EACH ATTRIBUTE Repealed
  53. 8.1Division 8.1 Repealed
  54. 8.2Division 8.2 Repealed
  55. 8.3Division 8.3 Repealed
  56. 8.4Division 8.4 Repealed
  57. 9SUPERVISION Repealed
  58. 10NOMENCLATURE Repealed
  59. 11REFERENCE Repealed
  60. Annex V: (Article 5) Repealed
  61. DETERMINATION OF THE CONTENT OF ENANTHIC ACID TRIGLYCERIDE IN BUTTER, BUTTER-OIL AND CREAM BY GAS CHROMATOGRAPHIC ANALYSIS OF TRIGLYCERIDES Repealed
  62. 1SCOPE Repealed
  63. 2TERMS AND DEFINITION Repealed
  64. 3PRINCIPLE Repealed
  65. REAGENTS Repealed
  66. Division Repealed
  67. 4.1Division 4.1 Repealed
  68. 4.2Division 4.2 Repealed
  69. 4.3Division 4.3 Repealed
  70. 4.4Division 4.4 Repealed
  71. APPARATUS Repealed
  72. Division Repealed
  73. 5.1Division 5.1 Repealed
  74. 5.2Division 5.2 Repealed
  75. 5.3Division 5.3 Repealed
  76. 5.4Division 5.4 Repealed
  77. 5.5Division 5.5 Repealed
  78. 5.6Division 5.6 Repealed
  79. Repealed
  80. Division Repealed
  81. 5.7.1Division 5.7.1 Repealed
  82. Repealed
  83. Division Repealed
  84. 5.7.2.1Division 5.7.2.1 Repealed
  85. 5.7.2.2Division 5.7.2.2 Repealed
  86. 5.8Division 5.8 Repealed
  87. 6SAMPLING Repealed
  88. PROCEDURE Repealed
  89. Preparation of the test sample and test portion Repealed
  90. Division Repealed
  91. Butter-oil, Butter Repealed
  92. 7.1.1.1Division 7.1.1.1 Repealed
  93. 7.1.1.2Division 7.1.1.2 Repealed
  94. 7.1.1.3Division 7.1.1.3 Repealed
  95. Cream Repealed
  96. 7.1.2.1Division 7.1.2.1 Repealed
  97. 7.1.2.2Division 7.1.2.2 Repealed
  98. 7.1.2.3Division 7.1.2.3 Repealed
  99. 7.1.2.4Division 7.1.2.4 Repealed
  100. 7.1.2.5Division 7.1.2.5 Repealed
  101. 7.1.2.6Division 7.1.2.6 Repealed
  102. Preparation of the calibration standards Repealed
  103. 7.2.1Division 7.2.1 Repealed
  104. 7.2.2Division 7.2.2 Repealed
  105. 7.2.3Division 7.2.3 Repealed
  106. 7.2.4Division 7.2.4 Repealed
  107. 7.2.5Division 7.2.5 Repealed
  108. Chromatographic determination Repealed
  109. 7.3.1Division 7.3.1 Repealed
  110. 7.3.2Division 7.3.2 Repealed
  111. 7.3.3Division 7.3.3 Repealed
  112. CALCULATION OF RESULTS Repealed
  113. Division Repealed
  114. Calibration Repealed
  115. 8.1.1Division 8.1.1 Repealed
  116. 8.1.2Division 8.1.2 Repealed
  117. 8.1.3Division 8.1.3 Repealed
  118. 8.2Test samples Repealed
  119. PRECISION Repealed
  120. Division Repealed
  121. 9.1Repeatability Repealed
  122. 9.2Reproducibility Repealed
  123. TOLERANCE LIMITS: LOWER LIMITS (CASE OF INSUFFICIENT QUANTITIES) Repealed
  124. 10.1Division 10.1 Repealed
  125. Butter and concentrated butter Repealed
  126. 10.2.1Division 10.2.1 Repealed
  127. 10.2.2Division 10.2.2 Repealed
  128. Cream Repealed
  129. 10.3.1Division 10.3.1 Repealed
  130. 10.3.2Division 10.3.2 Repealed
  131. TOLERANCE LIMITS: UPPER LIMITS (CASE OF EXCEEDING QUANTITY BY MORE THAN 20 %) Repealed
  132. 11.1Division 11.1 Repealed
  133. Butter and concentrated butter Repealed
  134. 11.2.1Division 11.2.1 Repealed
  135. Cream Repealed
  136. 11.3.1Division 11.3.1 Repealed
  137. ADDITIONAL INFORMATION: STATISTICAL ANALYSIS OF RESULTS ON THE DETERMINATION OF TRIENANTOATE IN BUTTERFAT BY TRIGLYCERIDE ANALYSIS Repealed
  138. Division Repealed
  139. Tests for outliers: Repealed
  140. Precision parameters Repealed
  141. Figure 1 Repealed
  142. Experimental results: Sample A Repealed
  143. Experimental results: Sample B Repealed
  144. Experimental results: Sample C Repealed
  145. Experimental results: Sample D Repealed
  146. Annex VI: (Article 5) Repealed
  147. DETERMINING THE VANILLIN CONTENT IN CONCENTRATED BUTTER, BUTTER OR CREAM BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Repealed
  148. 1SCOPE AND FIELD OF APPLICATION Repealed
  149. 2PRINCIPLE Repealed
  150. APPARATUS Repealed
  151. Division Repealed
  152. 3.1Division 3.1 Repealed
  153. 3.2Division 3.2 Repealed
  154. 3.3Division 3.3 Repealed
  155. 3.4Division 3.4 Repealed
  156. 3.5Division 3.5 Repealed
  157. 3.6Division 3.6 Repealed
  158. 3.7Division 3.7 Repealed
  159. REAGENTS Repealed
  160. Division Repealed
  161. 4.1Division 4.1 Repealed
  162. 4.2Division 4.2 Repealed
  163. 4.3Division 4.3 Repealed
  164. 4.4Division 4.4 Repealed
  165. Repealed
  166. Division Repealed
  167. 4.5.1Vanillin stock solution (= 500 μg/ml) Repealed
  168. 4.5.2Vanillin standard solution (= 10 μg/ml) Repealed
  169. 4.5.3Division 4.5.3 Repealed
  170. 4.5.4Division 4.5.4 Repealed
  171. 4.5.5Division 4.5.5 Repealed
  172. 4.5.6HPLC mobile phase Repealed
  173. PROCEDURE Repealed
  174. Preparation of the test sample Repealed
  175. 5.1.1Butter Repealed
  176. 5.1.2Concentrated butter Repealed
  177. 5.1.3Cream Repealed
  178. 5.2Preparation of the test solution Repealed
  179. 5.3Calibration Repealed
  180. 5.4Determination by HPLC Repealed
  181. 6CALCULATION OF THE RESULTS Repealed
  182. ACCURACY OF THE METHOD Repealed
  183. 7.1Repeatability (r) Repealed
  184. 7.2Reproducibility (R) Repealed
  185. TOLERANCE LIMITS Repealed
  186. 8.1Division 8.1 Repealed
  187. Repealed
  188. Division Repealed
  189. 8.2.1Division 8.2.1 Repealed
  190. 8.2.2Division 8.2.2 Repealed
  191. Repealed
  192. Division Repealed
  193. 8.3.1Division 8.3.1 Repealed
  194. 8.3.2Division 8.3.2 Repealed
  195. NOTES Repealed
  196. 9.1Division 9.1 Repealed
  197. 9.2Division 9.2 Repealed
  198. 9.3Division 9.3 Repealed
  199. 9.4Division 9.4 Repealed
  200. Annex VII: (Article 5) Repealed
  201. DETERMINING THE ETHYL ESTER OF BETA-APO-8'-CAROTENIC ACID IN CONCENTRATED BUTTER AND BUTTER BY SPECTROMETRY Repealed
  202. 1SCOPE AND FIELD OF APPLICATION Repealed
  203. 2PRINCIPLE Repealed
  204. APPARATUS Repealed
  205. 3.1Division 3.1 Repealed
  206. 3.2Division 3.2 Repealed
  207. 3.3Division 3.3 Repealed
  208. 3.4Division 3.4 Repealed
  209. 3.5Division 3.5 Repealed
  210. 3.6Division 3.6 Repealed
  211. REAGENTS Repealed
  212. Division Repealed
  213. Repealed
  214. Division Repealed
  215. 4.1.1Division 4.1.1 Repealed
  216. 4.1.2Division 4.1.2 Repealed
  217. 4.2Division 4.2 Repealed
  218. 4.3Division 4.3 Repealed
  219. 4.4Division 4.4 Repealed
  220. 4.5Division 4.5 Repealed
  221. PROCEDURE Repealed
  222. Preparation of the test sample Repealed
  223. 5.1.1Concentrated butter Repealed
  224. 5.1.2Butter Repealed
  225. 5.2Determination Repealed
  226. 5.3Calibration Repealed
  227. CALCULATION OF THE RESULTS Repealed
  228. 6.1Division 6.1 Repealed
  229. ACCURACY OF THE METHOD Repealed
  230. Repeatability Repealed
  231. 7.1.1Butter analysis Repealed
  232. 7.1.2Concentrated butter analysis Repealed
  233. Reproducibility Repealed
  234. 7.2.1Butter analysis Repealed
  235. 7.3Concentrated butter analysis Repealed
  236. 7.4Source of precision data Repealed
  237. TOLERANCE LIMITS Repealed
  238. 8.1Division 8.1 Repealed
  239. Butter Repealed
  240. 8.2.1Division 8.2.1 Repealed
  241. 8.2.2Division 8.2.2 Repealed
  242. Concentrated butter Repealed
  243. 8.3.1Division 8.3.1 Repealed
  244. Annex VIII: (Article 5) Repealed
  245. DETERMINING SITOSTEROL OR STIGMASTEROL IN BUTTER OR CONCENTRATED BUTTER BY CAPILLARY-COLUMN GAS CHROMATOGRAPHY Repealed
  246. 1SCOPE AND FIELD OF APPLICATION Repealed
  247. 2PRINCIPLE Repealed
  248. APPARATUS Repealed
  249. 3.1Division 3.1 Repealed
  250. 3.2Division 3.2 Repealed
  251. 3.3Division 3.3 Repealed
  252. 3.4Division 3.4 Repealed
  253. 3.5Division 3.5 Repealed
  254. 3.6Division 3.6 Repealed
  255. 3.7Division 3.7 Repealed
  256. Repealed
  257. Division Repealed
  258. 3.8.1Division 3.8.1 Repealed
  259. 3.8.2Division 3.8.2 Repealed
  260. 3.8.3Division 3.8.3 Repealed
  261. 3.8.4Division 3.8.4 Repealed
  262. 3.9Division 3.9 Repealed
  263. 3.10Division 3.10 Repealed
  264. REAGENTS Repealed
  265. Division Repealed
  266. 4.1Division 4.1 Repealed
  267. 4.2Division 4.2 Repealed
  268. Repealed
  269. Division Repealed
  270. Repealed
  271. Division Repealed
  272. 4.3.1.1Division 4.3.1.1 Repealed
  273. 4.3.1.2Division 4.3.1.2 Repealed
  274. 4.4Division 4.4 Repealed
  275. 4.5Division 4.5 Repealed
  276. 4.6Division 4.6 Repealed
  277. 4.7Division 4.7 Repealed
  278. Repealed
  279. Division Repealed
  280. 4.8.1Division 4.8.1 Repealed
  281. Repealed
  282. Division Repealed
  283. 4.9.1Division 4.9.1 Repealed
  284. 4.10Division 4.10 Repealed
  285. METHOD Repealed
  286. Preparation of standard solutions for chromatography Repealed
  287. Division Repealed
  288. 5.1.1Division 5.1.1 Repealed
  289. 5.1.2Division 5.1.2 Repealed
  290. Preparation of the unsaponifiables Repealed
  291. 5.2.1Division 5.2.1 Repealed
  292. 5.2.2Division 5.2.2 Repealed
  293. 5.2.3Division 5.2.3 Repealed
  294. Preparation of trimethyl silyl ethers Repealed
  295. 5.3.1Division 5.3.1 Repealed
  296. 5.3.2Division 5.3.2 Repealed
  297. Gas-chromatographic analysis Repealed
  298. 5.4.1Choice of operating conditions Repealed
  299. 5.4.2Analytical procedure Repealed
  300. CALCULATION OF RESULTS Repealed
  301. 6.1Division 6.1 Repealed
  302. ACCURACY OF THE METHOD Repealed
  303. Butter Repealed
  304. Repeatability Repealed
  305. 7.1.1.1Stigmasterol Repealed
  306. 7.1.1.2Sitosterol Repealed
  307. Reproducibility Repealed
  308. 7.1.2.1Stigmasterol Repealed
  309. 7.1.2.2Sitosterol Repealed
  310. 7.1.3Source of precision data Repealed
  311. Concentrated butter Repealed
  312. Repeatability Repealed
  313. 7.2.1.1Stigmasterol Repealed
  314. 7.2.1.2Sitosterol Repealed
  315. Reproducibility Repealed
  316. 7.2.2.1Stigmasterol Repealed
  317. 7.2.2.2Sitosterol Repealed
  318. 7.2.3Source of precision data Repealed
  319. TOLERANCE LIMITS Repealed
  320. 8.1Division 8.1 Repealed
  321. Butter Repealed
  322. Stigmasterol Repealed
  323. 8.2.1.1Division 8.2.1.1 Repealed
  324. 8.2.1.2Division 8.2.1.2 Repealed
  325. Sitosterol Repealed
  326. 8.2.2.1Division 8.2.2.1 Repealed
  327. 8.2.2.2Division 8.2.2.2 Repealed
  328. Concentrated butter Repealed
  329. Stigmasterol Repealed
  330. 8.3.1.1Division 8.3.1.1 Repealed
  331. 8.3.1.2Division 8.3.1.2 Repealed
  332. Sitosterol Repealed
  333. 8.3.2.1Division 8.3.2.1 Repealed
  334. 8.3.2.2Division 8.3.2.2 Repealed
  335. Division Repealed
  336. Division Repealed
  337. Annex IX: (Article 6) Repealed
  338. REFERENCE METHOD FOR THE DETECTION OF COWS’ MILK AND CASEINATE IN CHEESES FROM EWES’ MILK, GOATS’ MILK OR BUFFALOS’ MILK OR MIXTURES OF EWES’, GOATS’ AND BUFFALOS’ MILK Repealed
  339. 1SCOPE Repealed
  340. 2FIELD OF APPLICATION Repealed
  341. PRINCIPLE OF THE METHOD Repealed
  342. 3.1Division 3.1 Repealed
  343. 3.2Division 3.2 Repealed
  344. 3.3Division 3.3 Repealed
  345. 3.4Division 3.4 Repealed
  346. REAGENTS Repealed
  347. Division Repealed
  348. Isoelectric focusing Repealed
  349. Reagents for production of the urea containing polyacrylamide gels Repealed
  350. 4.1.1Stock gel solution Repealed
  351. 4.1.2Gel solution Repealed
  352. Catalyst solutions Repealed
  353. 4.1.3.1Division 4.1.3.1 Repealed
  354. 4.1.3.2Division 4.1.3.2 Repealed
  355. 4.2Contact fluid Repealed
  356. 4.3Anode solution Repealed
  357. 4.4Cathode solution Repealed
  358. Sample preparation Repealed
  359. Reagents for protein isolation Repealed
  360. 4.5.1Division 4.5.1 Repealed
  361. 4.5.2Division 4.5.2 Repealed
  362. 4.5.3Division 4.5.3 Repealed
  363. 4.6Protein dissolving buffer Repealed
  364. Reagents for plasmin cleavage of caseins Repealed
  365. 4.7.1Ammonium carbonate buffer Repealed
  366. 4.7.2Division 4.7.2 Repealed
  367. 4.7.3ε-Aminocaproic acid solution for enzyme inhibition Repealed
  368. Standards Repealed
  369. 4.8.1Division 4.8.1 Repealed
  370. 4.8.2Preparation of laboratory interim-standards of buffalos’ renneted milk containing 0 % and 1 % of cows’ milk Repealed
  371. Reagents for protein staining Repealed
  372. 4.9Fixative Repealed
  373. 4.10Destaining solution Repealed
  374. Staining solutions Repealed
  375. 4.11.1Staining solution (stock solution 1) Repealed
  376. 4.11.2Staining solution (stock solution 2) Repealed
  377. 4.11.3Staining solution (working solution) Repealed
  378. EQUIPMENT Repealed
  379. 5.1Division 5.1 Repealed
  380. 5.2Division 5.2 Repealed
  381. 5.3Division 5.3 Repealed
  382. 5.4Division 5.4 Repealed
  383. 5.5Division 5.5 Repealed
  384. 5.6Division 5.6 Repealed
  385. 5.7Division 5.7 Repealed
  386. 5.8Division 5.8 Repealed
  387. 5.9Division 5.9 Repealed
  388. 5.10Division 5.10 Repealed
  389. 5.11Division 5.11 Repealed
  390. 5.12Division 5.12 Repealed
  391. 5.13Division 5.13 Repealed
  392. 5.14Division 5.14 Repealed
  393. 5.15Division 5.15 Repealed
  394. 5.16Division 5.16 Repealed
  395. 5.17Division 5.17 Repealed
  396. 5.18Division 5.18 Repealed
  397. 5.19Division 5.19 Repealed
  398. PROCEDURE Repealed
  399. Sample preparation Repealed
  400. 6.1.1Isolation of caseins Repealed
  401. 6.1.2Plasmin cleavage of β-caseins to intensify γ-caseins Repealed
  402. 6.2Preparation of the urea containing polyacrylamide gels Repealed
  403. lsoelectric focusing Repealed
  404. Division Repealed
  405. Conditions for isoelectric focusing: Repealed
  406. 6.3.1Gel size 265 × 125 × 0,25 mm Repealed
  407. 6.3.2Example of a voltage programme for an automatic electrophoresis device (2 gels of 5,0 × 4,5 cm), electrodes without strips applied directly to the gel Repealed
  408. Protein staining Repealed
  409. 6.4.1Protein fixation Repealed
  410. 6.4.2Washing and staining the gel plate Repealed
  411. 6.4.3Destaining the gel plate Repealed
  412. EVALUATION Repealed
  413. Division Repealed
  414. 7.1Visual estimation Repealed
  415. 7.2Densitometric estimation Repealed
  416. 8REFERENCES Repealed
  417. Division Repealed
  418. Annex X: (Article 7) Repealed
  419. REFERENCE METHOD FOR DETECTING COLIFORMS IN BUTTER, SKIMMED-MILK POWDER, CASEIN AND CASEINATES Repealed
  420. 1PREPARATION OF SAMPLES Repealed
  421. 2PROCEDURE Repealed
  422. 3RESULTS Repealed
  423. Annex XI: (Article 8) Repealed
  424. DETERMINATION OF LACTOSE IN COMPOUND FEEDINGSTUFFS Repealed
  425. 1SCOPE AND FIELD OF APPLICATION Repealed
  426. 2REFERENCE Repealed
  427. 3DEFINITION Repealed
  428. 4PRINCIPLE Repealed
  429. REAGENTS Repealed
  430. 5.1General Repealed
  431. 5.2Lactose Repealed
  432. 5.3Concentrated Biggs/Szijarto solution Repealed
  433. 5.4Diluted Biggs/Szijarto solution Repealed
  434. 5.5Preparation of HPLC grade water Repealed
  435. APPARATUS Repealed
  436. Division Repealed
  437. 6.1HPLC ion exchange resin column Repealed
  438. 6.2Guard column Repealed
  439. 6.3Column oven Repealed
  440. 6.4HPLC pump Repealed
  441. 6.5HPLC injection device Repealed
  442. 6.6HPLC detector Repealed
  443. 6.7Integrator Repealed
  444. 6.8Water purification unit Repealed
  445. 6.9Solvent filtration unit Repealed
  446. 6.10Analytical balance Repealed
  447. 6.11Water bath Repealed
  448. 6.12Centrifuge Repealed
  449. 6.13Volumetric flask 50 mL Repealed
  450. 6.14Volumetric flask 100 mL Repealed
  451. 6.15Graduated pipette Repealed
  452. 7SAMPLING Repealed
  453. PREPARATION OF LACTOSE STANDARD SOLUTION Repealed
  454. 8.1Standard 1 Repealed
  455. 8.2Standard 2 Repealed
  456. PREPARATION OF THE TEST SAMPLE Repealed
  457. 9.1Reconstitution of the sample Repealed
  458. 9.2Sample treatment Repealed
  459. HPLC DETERMINATION Repealed
  460. Preliminary preparation of HPLC Repealed
  461. 10.1.1Installation of the column and pre-column Repealed
  462. 10.1.2Detector and initial flow Repealed
  463. 10.1.3Column oven and final flow-rate Repealed
  464. 10.1.4Integration Repealed
  465. 10.1.5Guard column test Repealed
  466. 10.2Running standards Repealed
  467. 10.3Running samples Repealed
  468. CALCULATION AND EXPRESSION OF THE RESULTS Repealed
  469. 11.1Calibration Repealed
  470. 11.2Samples Repealed
  471. PRECISION Repealed
  472. Division Repealed
  473. 12.1Repeatability Repealed
  474. 12.2Reproducibility Repealed
  475. REFERENCES Repealed
  476. Annex XII: (Article 9) Repealed
  477. DETECTION OF RENNET WHEY IN SKIMMED-MILK POWDER FOR PUBLIC STORAGE BY DETERMINATION OF CASEINOMACROPEPTIDES HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) Repealed
  478. 1SCOPE AND FIELD OF APPLICATION Repealed
  479. 2REFERENCE Repealed
  480. 3DEFINITION Repealed
  481. 4PRINCIPLE Repealed
  482. REAGENTS Repealed
  483. Division Repealed
  484. 5.1Trichloroacetic acid solution Repealed
  485. 5.2Eluent solution, pH 6,0 Repealed
  486. 5.3Flushing solvent Repealed
  487. Standard samples Repealed
  488. 5.4.1Division 5.4.1 Repealed
  489. 5.4.2Division 5.4.2 Repealed
  490. APPARATUS Repealed
  491. 6.1Division 6.1 Repealed
  492. 6.2Division 6.2 Repealed
  493. 6.3Division 6.3 Repealed
  494. 6.4Division 6.4 Repealed
  495. 6.5Division 6.5 Repealed
  496. 6.6Division 6.6 Repealed
  497. 6.7Division 6.7 Repealed
  498. 6.8Division 6.8 Repealed
  499. 6.9Division 6.9 Repealed
  500. 6.10Division 6.10 Repealed
  501. Repealed
  502. Division Repealed
  503. 6.11.1Division 6.11.1 Repealed
  504. 6.11.2Division 6.11.2 Repealed
  505. 6.11.3Division 6.11.3 Repealed
  506. 6.11.4Division 6.11.4 Repealed
  507. 6.11.5Division 6.11.5 Repealed
  508. 6.11.6Division 6.11.6 Repealed
  509. SAMPLING Repealed
  510. 7.1Division 7.1 Repealed
  511. 7.2Division 7.2 Repealed
  512. PROCEDURE Repealed
  513. 8.1Preparation of the test sample Repealed
  514. 8.2Test portion Repealed
  515. Removal of fat and proteins Repealed
  516. 8.3.1Division 8.3.1 Repealed
  517. 8.3.2Division 8.3.2 Repealed
  518. 8.3.3Division 8.3.3 Repealed
  519. Chromatographic determination Repealed
  520. 8.4.1Division 8.4.1 Repealed
  521. 8.4.2Division 8.4.2 Repealed
  522. Calibration Repealed
  523. 8.5.1Division 8.5.1 Repealed
  524. 8.5.2Division 8.5.2 Repealed
  525. 8.5.3Division 8.5.3 Repealed
  526. EXPRESSION OF RESULTS Repealed
  527. Method of calculation and formulae Repealed
  528. 9.1.1Calculation of the response factors R: Repealed
  529. 9.1.2Calculation of the relative area of the peaks in the sample [E] Repealed
  530. 9.1.3Calculation of the relative retention time of peak III in sample [E]: RRTIII[E] = (RTIII[E])/(RTIII[5]) Repealed
  531. 9.1.4Experiments have shown that there is a linear relation between the relative retention time of peak III, i.e. RRTIII [E] and the percentage of whey powder added up to 10 % Repealed
  532. 9.2Calculation of the percentage of rennet whey powder in the sample: Repealed
  533. Accuracy of the procedure Repealed
  534. 9.3.1Repeatability Repealed
  535. 9.3.2Reproducibility Repealed
  536. Interpretation Repealed
  537. 9.4.1Division 9.4.1 Repealed
  538. 9.4.2Division 9.4.2 Repealed
  539. Repealed
  540. Division Repealed
  541. 9.4.3.1Division 9.4.3.1 Repealed
  542. 9.4.3.2Division 9.4.3.2 Repealed
  543. Skimmed-milk powder Repealed
  544. Skimmed-milk powder Repealed
  545. Annex XIII: (Article 9) Repealed
  546. DETERMINING RENNET WHEY SOLIDS IN SKIMMED-MILK POWDER AND THE MIXTURES REFERRED TO IN REGULATION (EC) No 2799/1999 Repealed
  547. 1PURPOSE: DETECTING THE ADDITION OF RENNET WHEY SOLIDS TO: Repealed
  548. REFERENCES: INTERNATIONAL STANDARD ISO 707 Repealed
  549. 3DEFINITION Repealed
  550. 4PRINCIPLE Repealed
  551. REAGENTS Repealed
  552. Division Repealed
  553. 5.1Trichloroacetic acid solution Repealed
  554. 5.2Eluents A and B Repealed
  555. 5.3Conservation of the column Repealed
  556. Standard samples Repealed
  557. 5.4.1Division 5.4.1 Repealed
  558. 5.4.2Division 5.4.2 Repealed
  559. 5.4.3Division 5.4.3 Repealed
  560. APPARATUS Repealed
  561. Division Repealed
  562. 6.1Division 6.1 Repealed
  563. 6.2Division 6.2 Repealed
  564. 6.3Division 6.3 Repealed
  565. 6.4Division 6.4 Repealed
  566. 6.5Division 6.5 Repealed
  567. 6.6Division 6.6 Repealed
  568. 6.7Division 6.7 Repealed
  569. 6.8Division 6.8 Repealed
  570. 6.9Division 6.9 Repealed
  571. 6.10Division 6.10 Repealed
  572. Repealed
  573. Division Repealed
  574. 6.11.1Division 6.11.1 Repealed
  575. 6.11.2Division 6.11.2 Repealed
  576. 6.11.3Division 6.11.3 Repealed
  577. 6.11.4Division 6.11.4 Repealed
  578. 6.11.5Division 6.11.5 Repealed
  579. 6.11.6Division 6.11.6 Repealed
  580. SAMPLING Repealed
  581. 7.1Division 7.1 Repealed
  582. 7.2Division 7.2 Repealed
  583. PROCEDURE Repealed
  584. 8.1Preparation of the test sample Repealed
  585. 8.2Test portion Repealed
  586. Removal of fat and proteins Repealed
  587. 8.3.1Division 8.3.1 Repealed
  588. 8.3.2Division 8.3.2 Repealed
  589. 8.3.3Division 8.3.3 Repealed
  590. Chromatographic determination Repealed
  591. 8.4.1Division 8.4.1 Repealed
  592. 8.4.2Division 8.4.2 Repealed
  593. 8.4.3Division 8.4.3 Repealed
  594. 8.4.4Division 8.4.4 Repealed
  595. Calibration Repealed
  596. 8.5.1Division 8.5.1 Repealed
  597. 8.5.2Division 8.5.2 Repealed
  598. 8.5.3Division 8.5.3 Repealed
  599. EXPRESSION OF RESULTS Repealed
  600. Method of calculation and formulae Repealed
  601. 9.1.1Calculation of the response factor R: Repealed
  602. 9.2Calculation of the percentage of rennet whey powder in the sample Repealed
  603. Accuracy of the procedure Repealed
  604. 9.3.1Repeatability Repealed
  605. 9.3.2Reproducibility Repealed
  606. 9.3.3Linearity Repealed
  607. Interpretation Repealed
  608. Division Repealed
  609. Ni –4,6 standard Repealed
  610. Annex XIV: (Article 10) Repealed
  611. SKIMMED-MILK POWDER: QUANTITATIVE DETERMINATION OF PHOSPHATIDYLSERINE AND PHOSPHATIDYLETHANOLAMINE Repealed
  612. Division Repealed
  613. 1PURPOSE AND FIELD OF APPLICATION Repealed
  614. 2DEFINITION Repealed
  615. 3PRINCIPLE OF THE METHOD Repealed
  616. REAGENTS Repealed
  617. Division Repealed
  618. Standard material: PEDP, at least 99 % pure Repealed
  619. Reagents for standard sample and test sample preparation Repealed
  620. 4.2.1Division 4.2.1 Repealed
  621. 4.2.2Division 4.2.2 Repealed
  622. 4.2.3Division 4.2.3 Repealed
  623. Reagents for o-phthaldialdehyde derivatisation Repealed
  624. 4.3.1Division 4.3.1 Repealed
  625. 4.3.2Division 4.3.2 Repealed
  626. 4.3.3Division 4.3.3 Repealed
  627. 4.3.4Division 4.3.4 Repealed
  628. HPLC elution solvents Repealed
  629. 4.4.1Division 4.4.1 Repealed
  630. 4.4.2Division 4.4.2 Repealed
  631. 4.4.3Division 4.4.3 Repealed
  632. 4.4.4Division 4.4.4 Repealed
  633. 4.4.5Division 4.4.5 Repealed
  634. 4.4.6Division 4.4.6 Repealed
  635. 4.4.7Division 4.4.7 Repealed
  636. APPARATUS Repealed
  637. 5.1Division 5.1 Repealed
  638. 5.2Division 5.2 Repealed
  639. 5.3Division 5.3 Repealed
  640. 5.4Division 5.4 Repealed
  641. 5.5Division 5.5 Repealed
  642. 5.6Division 5.6 Repealed
  643. 5.7Division 5.7 Repealed
  644. 5.8Division 5.8 Repealed
  645. 5.9Division 5.9 Repealed
  646. 5.10Division 5.10 Repealed
  647. 5.11Division 5.11 Repealed
  648. 5.12Division 5.12 Repealed
  649. Repealed
  650. Division Repealed
  651. 5.13.1Division 5.13.1 Repealed
  652. 5.13.2Division 5.13.2 Repealed
  653. 5.13.3Division 5.13.3 Repealed
  654. 5.13.4Division 5.13.4 Repealed
  655. 5.13.5Division 5.13.5 Repealed
  656. 5.13.6Division 5.13.6 Repealed
  657. 6SAMPLING Repealed
  658. PROCEDURE Repealed
  659. Preparation of the internal standard solution Repealed
  660. 7.1.1Division 7.1.1 Repealed
  661. 7.1.2Division 7.1.2 Repealed
  662. Preparation of the test sample solution Repealed
  663. 7.2.1Division 7.2.1 Repealed
  664. 7.2.2Division 7.2.2 Repealed
  665. 7.2.3Division 7.2.3 Repealed
  666. Preparation of the external standard solution Repealed
  667. 7.3.1Division 7.3.1 Repealed
  668. 7.3.2Division 7.3.2 Repealed
  669. 7.4Preparation of the derivatising reagent Repealed
  670. Determination by HPLC Repealed
  671. 7.5.1Elution solvents (4.4) Repealed
  672. 7.5.2Suggested eluting gradient: Repealed
  673. 7.5.3Division 7.5.3 Repealed
  674. 7.5.4Column equilibration Repealed
  675. 7.5.5Division 7.5.5 Repealed
  676. Perform the sequence of the chromatographic analyses keeping constant the run-to-run time in order to obtain constant retention times. Inject the external standard solution (7.3) every 5-10 test sample solutions in order to calculate the response factor 0 Repealed
  677. Integration mode Repealed
  678. 7.6.1PEDP peak Repealed
  679. 7.6.2Tryptamine peak Repealed
  680. 7.6.3PS and PE peaks groups Repealed
  681. 8CALCULATION AND EXPRESSION OF RESULTS Repealed
  682. ACCURACY OF THE METHOD Repealed
  683. Repealed
  684. 9.1Repeatability Repealed
  685. 9.2Reproducibility Repealed
  686. REFERENCES Repealed
  687. 10.1Division 10.1 Repealed
  688. Annex XV: (Article 11) Repealed
  689. DETECTION OF ANTIMICROBIAL RESIDUES IN SKIMMED MILK POWDER Repealed
  690. Division Repealed
  691. Positive results are to be interpreted as follows: Repealed
  692. Annex XVI: (Article 12) Repealed
  693. QUANTITATIVE DETERMINATION OF SKIMMED-MILK POWDER IN COMPOUND FEEDINGSTUFFS BY ENZYMATIC COAGULATION OF PARA-CASEIN Repealed
  694. 1PURPOSE Repealed
  695. 2SCOPE Repealed
  696. PRINCIPLE OF THE METHOD Repealed
  697. 3.1Division 3.1 Repealed
  698. 3.2Division 3.2 Repealed
  699. 3.3Division 3.3 Repealed
  700. REAGENTS Repealed
  701. Division Repealed
  702. 4.1Division 4.1 Repealed
  703. 4.2Division 4.2 Repealed
  704. 4.3Division 4.3 Repealed
  705. 4.4Division 4.4 Repealed
  706. 4.5Division 4.5 Repealed
  707. 4.6Division 4.6 Repealed
  708. APPARATUS Repealed
  709. Division Repealed
  710. 5.1Division 5.1 Repealed
  711. 5.2Division 5.2 Repealed
  712. 5.3Division 5.3 Repealed
  713. 5.4Division 5.4 Repealed
  714. 5.5Division 5.5 Repealed
  715. 5.6Division 5.6 Repealed
  716. 5.7Division 5.7 Repealed
  717. 5.8Division 5.8 Repealed
  718. 5.9Division 5.9 Repealed
  719. 5.10Division 5.10 Repealed
  720. 5.11Division 5.11 Repealed
  721. 5.12Division 5.12 Repealed
  722. PROCEDURE Repealed
  723. 6.1Preparation of the sample Repealed
  724. Dissolving of milk powder and separation of the insoluble residue Repealed
  725. 6.2.1Division 6.2.1 Repealed
  726. 6.2.2Division 6.2.2 Repealed
  727. 6.2.3Division 6.2.3 Repealed
  728. 6.2.4Division 6.2.4 Repealed
  729. Coagulation of casein with the enzymes of rennet Repealed
  730. 6.3.1Division 6.3.1 Repealed
  731. 6.3.2Division 6.3.2 Repealed
  732. 6.3.3Division 6.3.3 Repealed
  733. 6.3.4Division 6.3.4 Repealed
  734. Determination of casein nitrogen Repealed
  735. 6.4.1Division 6.4.1 Repealed
  736. BLANK TEST Repealed
  737. 7.1Division 7.1 Repealed
  738. 7.2Division 7.2 Repealed
  739. EXPRESSION OF RESULTS Repealed
  740. 8.1Division 8.1 Repealed
  741. ACCURACY OF THE METHOD Repealed
  742. 9.1Repeatability Repealed
  743. 9.2Reproducibility Repealed
  744. OBSERVATIONS Repealed
  745. 10.1Division 10.1 Repealed
  746. 10.2Division 10.2 Repealed
  747. 10.3Division 10.3 Repealed
  748. 10.4Division 10.4 Repealed
  749. Annex XVII: (Article 13) Repealed
  750. DETECTION OF STARCH IN SKIMMED-MILK POWDER, DENATURED MILK POWDER AND COMPOUND FEEDINGSTUFFS Repealed
  751. 1SCOPE Repealed
  752. 2PRINCIPLE Repealed
  753. REAGENTS Repealed
  754. 3.1Division 3.1 Repealed
  755. APPARATUS Repealed
  756. 4.1Division 4.1 Repealed
  757. 4.2Division 4.2 Repealed
  758. 4.3Division 4.3 Repealed
  759. 5PROCEDURE Repealed
  760. 6EXPRESSION OF RESULTS Repealed
  761. 7REMARKS Repealed
  762. Annex XVIII: (Article 14) Repealed
  763. DETERMINATION OF MOISTURE CONTENT IN DRIED CREAM Repealed
  764. 1SCOPE Repealed
  765. 2TERMS AND DEFINITIONS Repealed
  766. 3PRINCIPLE Repealed
  767. APPARATUS Repealed
  768. Division Repealed
  769. 4.1Division 4.1 Repealed
  770. 4.2Division 4.2 Repealed
  771. 4.3Division 4.3 Repealed
  772. 4.4Division 4.4 Repealed
  773. 4.5Division 4.5 Repealed
  774. 5SAMPLING Repealed
  775. 6PREPARATION OF TEST SAMPLE Repealed
  776. PROCEDURE Repealed
  777. Preparation of the dish Repealed
  778. 7.1.1Division 7.1.1 Repealed
  779. 7.1.2Division 7.1.2 Repealed
  780. 7.2Test portion Repealed
  781. Determination Repealed
  782. 7.3.1Division 7.3.1 Repealed
  783. 7.3.2Division 7.3.2 Repealed
  784. 7.3.3Division 7.3.3 Repealed
  785. 7.3.4Division 7.3.4 Repealed
  786. CALCULATION AND EXPRESSION OF RESULTS Repealed
  787. 8.1Calculation Repealed
  788. PRECISION Repealed
  789. Repealed
  790. 9.1Repeatability Repealed
  791. 9.2Reproducibility Repealed
  792. 10TEST REPORT Repealed
  793. Annex XIX: (Article 15) Repealed
  794. DETERMINATION OF MOISTURE IN ACID BUTTERMILK POWDER Repealed
  795. 1SCOPE Repealed
  796. 2PRINCIPLE Repealed
  797. APPARATUS Repealed
  798. 3.1Division 3.1 Repealed
  799. 3.2Division 3.2 Repealed
  800. 3.3Division 3.3 Repealed
  801. 3.4Division 3.4 Repealed
  802. 3.5Division 3.5 Repealed
  803. 4PROCEDURE Repealed
  804. 5CALCULATION Repealed
  805. PRECISION Repealed
  806. 6.1Repeatability limit Repealed
  807. 6.2Reproducibility limit Repealed
  808. 6.3Source of precision data Repealed
  809. Annex XX: (Article 16) Repealed
  810. REFERENCE METHOD FOR THE DETERMINATION OF MILK FAT PURITY BY GAS CHROMATOGRAPHIC ANALYSIS OF TRIGLYCERDIES — REVISION 2 Repealed
  811. 1SCOPE AND FIELD OF APPLICATION Repealed
  812. 2DEFINITION Repealed
  813. 3PRINCIPLE OF THE METHOD Repealed
  814. REAGENTS Repealed
  815. Division Repealed
  816. 4.1Division 4.1 Repealed
  817. Repealed
  818. Division Repealed
  819. 4.2.1Division 4.2.1 Repealed
  820. 4.2.2Division 4.2.2 Repealed
  821. 4.3Division 4.3 Repealed
  822. 4.4Division 4.4 Repealed
  823. 4.5Division 4.5 Repealed
  824. 4.6Division 4.6 Repealed
  825. 4.7Division 4.7 Repealed
  826. APPARATUS Repealed
  827. Division Repealed
  828. 5.1High-temperature gas chromatograph Repealed
  829. Chromatography column Repealed
  830. 5.2.1Packed column Repealed
  831. 5.2.2Capillary column Repealed
  832. 5.3Division 5.3 Repealed
  833. 5.4Division 5.4 Repealed
  834. 5.5Division 5.5 Repealed
  835. 5.6Division 5.6 Repealed
  836. 5.7Division 5.7 Repealed
  837. 5.8Division 5.8 Repealed
  838. 5.9Division 5.9 Repealed
  839. 5.10Division 5.10 Repealed
  840. 5.11Division 5.11 Repealed
  841. 5.12Division 5.12 Repealed
  842. 5.13Division 5.13 Repealed
  843. 5.14Division 5.14 Repealed
  844. 5.15Division 5.15 Repealed
  845. 5.16Division 5.16 Repealed
  846. 6SAMPLING Repealed
  847. PROCEDURE Repealed
  848. Preparation of test samples Repealed
  849. Division Repealed
  850. 7.1.1Isolation from butter or butteroil Repealed
  851. 7.1.2Extraction according to the Röse–Gottlieb gravimetric method Repealed
  852. 7.1.3Extraction from milk using silica gel columns Repealed
  853. 7.2Preparation of sample solution Repealed
  854. Chromatographic triglyceride determination Repealed
  855. 7.3.1Baseline drift Repealed
  856. Injection technique Repealed
  857. 7.3.2.1Packed column Repealed
  858. 7.3.2.2Capillary column Repealed
  859. Calibration Repealed
  860. 7.3.3.1General Repealed
  861. 7.3.3.2Commercial milk fat standard Repealed
  862. 7.3.3.3Laboratory milk fat standard Repealed
  863. Chromatographic conditions Repealed
  864. Repealed
  865. 7.3.4.1Packed column Repealed
  866. 7.3.4.2Capillary column Repealed
  867. 8INTEGRATION, EVALUATION AND CONTROL OF THE ANALYTICAL PERFORMANCE Repealed
  868. Division Repealed
  869. CALCULATION AND EXPRESSION OF RESULTS Repealed
  870. Triglyceride composition Repealed
  871. 9.1.1Calculation Repealed
  872. 9.1.2Expression of test results Repealed
  873. S-values Repealed
  874. Calculation Repealed
  875. 9.2.1.1Division 9.2.1.1 Repealed
  876. 9.2.1.2Division 9.2.1.2 Repealed
  877. 9.2.1.3Division 9.2.1.3 Repealed
  878. 9.2.1.4Division 9.2.1.4 Repealed
  879. 9.2.1.5Division 9.2.1.5 Repealed
  880. 9.2.1.6Division 9.2.1.6 Repealed
  881. 9.2.2Expression of test results Repealed
  882. 9.3Detection of foreign fat Repealed
  883. PRECISION Repealed
  884. 10.1Interlaboratory test Repealed
  885. 10.2Repeatability Repealed
  886. 10.3Reproducibility Repealed
  887. 11UNCERTAINTY OF MEASUREMENT Repealed
  888. 12TEST REPORT Repealed
  889. ANNEX A(normative) Repealed
  890. ANNEX B(informative) Repealed
  891. Annex XXI: (Article 18) Repealed
  892. PROCEDURE APPLICABLE WHEN THE RESULTS OF AN ANALYSIS ARE DISPUTED (CHEMICAL ANALYSIS) Repealed
  893. 1Division 1 Repealed
  894. 2Division 2 Repealed
  895. 3Division 3 Repealed
  896. 4Division 4 Repealed
  897. Annex XXIICORRELATION TABLE Repealed