Division 3 Reagents

United Kingdom

1. 3.1Division 3.1
2. 3.2Division 3.2
3. 3.3Division 3.3
4. 3.4Division 3.4
5. 3.5Division 3.5
6. 3.6Division 3.6
7. 3.7Division 3.7
8. 3.8Division 3.8
9. 3.9Division 3.9
10. 3.10Division 3.10
11. 3.11Division 3.11
12. 3.12Division 3.12
13. 3.13Division 3.13
14. 3.14Division 3.14
15. 3.15Division 3.15
16. 3.16Division 3.16
17. 3.16.1Division 3.16.1
18. 3.16.2Division 3.16.2
19. 3.16.3Division 3.16.3
20. 3.17Sodium hydroxide solution, c = 7,5 mol/l:
21. 3.18Sodium hydroxide solution, c = 1 mol/l:
22. 3.19Formic acid — phenol solution:
23. 3.20Hydrolysis mixture, c = 6 mol HCl/l containing 1 g phenol/l:
24. 3.21Division 3.21
25. 3.225-Sulfosalicylic acid, ß = 6 %:
26. 3.23Oxidation mixture (Performic acid — phenol):
27. 3.24Citrate buffer, c = 0,2 mol Na+/l, pH 2,2:
28. 3.25Division 3.25
29. 3.26Division 3.26
30. 3.27Division 3.27
31. 3.27.1Stock standard solution of amino acids (3.16.1).
32. 3.27.2Stock standard solution of cysteic acid and methionine sulphone, c = 1,25 μmol/ml.
33. 3.27.3Stock standard solution of the internal standard e.g. norleucine, c = 20 μmol/ml.
34. 3.27.4Calibration solution of standard amino acids for use with hydrolysates, c = 5 nmol/50 μl of cysteic acid and methionine sulphone and c = 10 nmol/50 μl of the other amino acids. Dissolve 2,2 g sodium chloride (3.10) in 100 ml beaker with 30 ml citrate buffer (3.24). Add 4,0 ml stock standard solution of amino acids (3.27.1), 4,0 ml stock standard solution of cysteic acid and methionine sulphone (3.27.2) and 0,5 ml stock standard solution of internal standard (3.27.3) if used. Adjust pH to 2,2 with sodium hydroxide (3.18).
35. 3.27.5Calibration solution of standard amino acids for use with hydrolysates prepared according to paragraph 5.3.3.1 and for use with extracts (5.2). The calibration solution is prepared according to 3.27.4 but omitting sodium chloride.