Division 3.27.4 Calibration solution of standard amino acids for use with hydrolysates, c = 5 nmol/50 μl of cysteic acid and methionine sulphone and c = 10 nmol/50 μl of the other amino acids. Dissolve 2,2 g sodium chloride (3.10) in 100 ml beaker with 30 ml citrate buffer (3.24). Add 4,0 ml stock standard solution of amino acids (3.27.1), 4,0 ml stock standard solution of cysteic acid and methionine sulphone (3.27.2) and 0,5 ml stock standard solution of internal standard (3.27.3) if used. Adjust pH to 2,2 with sodium hydroxide (3.18).

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