Division 5.3.4 Sample solution for chromatography

Quantitatively transfer the pH adjusted hydrolysate (5.3.3.1 or 5.3.3.2) with citrate buffer (3.24) to a 200 ml graduated flask, and make up to the mark with buffer (3.24).

If an internal standard has not already been used, add 2,0 ml of internal standard (3.27.3) and make up to the mark with citrate buffer (3.24). Mix thoroughly.

Proceed to the chromatography step (5.4).

If the sample solutions are not being examined the same day they must be stored below 5 ^oC.