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Division 6 Calculation of results

United Kingdom

Original text. Later amendments are not shown.

The area of the sample and standard peaks is measured for each individual amino acid and the amount (X), in g amino acid per kg sample, is calculated as follows:

X=A×c×M×VB×m×1 000

If an internal standard is used multiply by: DC

A

  • peak area, hydrolysate or extract

    • B

  • peak area, calibration standard solution

    • C

  • peak area, internal standard in hydrolysate or extract

    • D

  • peak area, internal standard, calibration standard solution

    • M

  • molar weight of the amino acid being determined

    • c

  • concentration of standard in μmol/ml

    • m

  • sample weight (g) (corrected to original weight if dried or defatted)

    • V

  • ml total hydrolysate (5.3.4) or ml calculated total dilution volume of extract (6.1)

    • Cystine and cysteine are both determined as cysteic acid in hydrolysates of oxidised sample, but calculated as cystine (C6H12N2O4S2, M 240,3 g/mol) by using M 120,15 g/mol (= 0,5 x 240,3 g/mol).

    Methionine is determined as methionine sulphone in hydrolysates of oxidised sample, but calculated as methionine by using M of methionine: 149,21 g/mol.

    Added free methionine is determined after extraction as methionine, for the calculation the same M is used.

    1. 6.1The total dilution volume of extracts (F) for determination of free amino acids (5.2) is calculated as follows: