Division 9.1 Following special chromatographic conditions may give better separation between tryptophan and α-methyl-tryptophan.
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Isocratic elution followed by gradient column cleaning:
| Liquid chromatographic column: | 125 mm x 4 mm, C18, 5 μm packing or equivalent | ||
| Column temperature: | 32 oC | ||
| Mobile phase: | A: 0,01 mol/l KH2PO4/méthanol, 95+5 (V+V). B: methanol | ||
| Gradient program: | 0 min. | 100 % A | 0 % B |
| 15 min. | 100 % A | 0 % B | |
| 17 min. | 60 % A | 40 % B | |
| 19 min. | 60 % A | 40 % B | |
| 21 min. | 100 % A | 0 % B | |
| 33 min. | 100 % A | 0 % B | |
| Flow rate: | 1,2 ml/min. | ||
| Total run time: | approx. 33 min. | ||