Division 5.3.1 Parameters
United Kingdom
The following conditions are offered for guidance; other conditions may be used, provided they yield equivalent results:
| Liquid chromatographic column (4.3.1): | 125 mm × 4 mm, reversed-phase C18, 5 μm packing or equivalent |
| Mobile phase (3.9): | Mixture of phosphate buffer solution (3.7) and methanol (3.5), 5+95 (V+V) |
| Flow rate: | 1,2 ml/min. |
| Detection wavelengths: | |
| Excitation: | 310 nm |
| Emission: | 419 nm |
| Injection volume: | 20 μl |
Check the stability of the chromatographic system, injecting the calibration solution (3.10.3) containing 4,0 μg/ml several times, until constant peak heights (or areas) and retention times are achieved.