Division 5.3.1 Parameters:
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The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.
| Liquid chromatographic | |
| column (4.1.1): | 125 mm × 4 mm, cation exchange Nucleosil 10 SA, 5 or 10 μm packing, or equivalent |
| Mobile phase (3.6): | Mixture of acetonitrile (3.2), sodium dihydrogen phosphate solution (3.4) and sodium perchlorate solution (3.5), 450+450+100 (v+v+v). |
| Flow rate: | 0,7-1 ml/min |
| Detection wavelength: | 264 nm |
| Injection volume: | 100 μl |
Check the stability of the chromatographic system, injecting several times the calibration solution (3.7.3) containing 1,0 μg/ml, until constant peak heights and retention times are achieved.