Division 5.4.1 Parameters
United Kingdom
The following conditions are offered for guidance, other conditions may be used provided they yield equivalent results:
| Liquid chromatographic | |
| column (4.4.1): | 300 mm × 4 mm, C18, 10 μmpacking or equivalent |
| Mobile phase (3.10): | Mixture of acetate buffer solution (3.9) and acetonitrile (3.2), 825 + 175 (v+v) |
| Flow rate: | 1,5-2 ml/min. |
| Detection wavelength: | 365 nm |
| Injection volume: | 20 μl |
Check the stability of the chromatographic system, injecting the calibration solution (3.11.2) containing 5,0 μg/ml several times, until constant peak heights (areas) and retention times are achieved.