Division 4.2.3 PCR mix
United Kingdom
PCR mix for each tube is:
| Volume per tube | Final concentration | |
|---|---|---|
| Buffer (10 X) | 5 μl | 1 X |
| MgCl2 (25 mM) | 5 μl | 2,5 mM |
| dNTP (2 mM) | 5 μl | 0,2 mM |
| CF (10 μM) | 1 μl | 0,2 μM |
| CR (10 μM) | 1 μl | 0,2 μM |
| Taq polymérase (5U/μl) | 0,5 μl | 2,5 U |
| dH2O | 31,5 μl | |
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49 μl of this PCR mix is dispensed in each PCR tube
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1 μl of extracted DNA (50-100 ng/μl) is added to each tube