Division 4.3.1 Reactives

50 X TAE (can be bought directly ready for use):

• Tris base (40 mM) 242 g

• Acetic glacial acid (40 mM) 57,1 ml

• Na₂EDTA.2H₂O (1 mM) 18,61 g

• dH₂O for 1 liter

• Ajust at pH 8

Agarose gel 2,5 % in 1 X TAE

Ethidium bromide (0,5 μg/ml) added after cooling the gel.

Loading blue dye:

• Bromophenol blue 0,25 %

• Cyanol xylene FF 0,25 %

• Sucrose 40 %

• Keep at 4 °C.

• Use diluted 6 times (2 μl of loading blue buffer for 10μl of PCR products).

Molecular weight marker:

SmartLadder SF (Eurogentec): a ready-to-use molecular weight marker including 9 regularly spaced bands from 100 to 1 000 bp.