Division 4.3.2 Agarose gel preparation

Weight 2,5 g of agarose, add 100 ml of 1 X TAE and heat until the mix is melted.

After cooling the solution, ethidium bromide is added (5 μl for 100 ml of agarose gel) and the solution is disposed in a specific mould equipped with combs (to form slots).

When gel is polymerised, combs are removed and gel is placed in a horizontal electrophoresis system containing enough 1 X TAE to the cover agarose gel.

10 μl of PCR products are mixed with 2 μl of blue dye (6 X) and disposed in the slots.

One whole is dedicated to the molecular weight marker (5 μl).

A voltage of 50 to 150 volts is applied during 30 min to 1 hour depending on the gel size and thickness.

Gel is observed under UV.