Schedule 4 ANALYTICAL METHODOLOGY
TABLE A1
PARAMETERS FOR WHICH, SUBJECT TO REGULATION 16(7),METHODS OF ANALYSIS ARE PRESCRIBED
| (1) | (2) | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Parameter | Method | ||||||||||||||||||||||||||||
* The composition of m-CP agar is:
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
| |||||||||||||||||||||||||||||
| Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agarf1 at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. | ||||||||||||||||||||||||||||
| Coliform bacteria | ISO 9308-1 | ||||||||||||||||||||||||||||
| Colony count 22°C-enumeration of culturable microorganisms | PrEN ISO 6222 | ||||||||||||||||||||||||||||
| Colony count 37°C-enumeration of culturable microoganisms | prEN ISO 6222 | ||||||||||||||||||||||||||||
| Enterococci | ISO 7899-2 | ||||||||||||||||||||||||||||
| Escherichia coli (E. coli) | ISO 9308-1 | ||||||||||||||||||||||||||||
TABLE A2
PARAMETERS IN RELATION TO WHICH METHODS OF ANALYSIS MUSTSATISFY PRESCRIBED CHARACTERISTICS
| (1) | (2) | (3) | (4) |
|---|---|---|---|
| Parameters | Trueness % of prescribed concentration or value or specification | Precision % of prescribed concentration or value or specification | Limit of detection % of prescribed concentration or value or specification |
| Notes: (i) The method of analysis should determine total cyanide in all forms. | |||
(ii) The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. | |||
(iii) The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1. | |||
(iv) The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1. | |||
(v) The performance characteristics apply to the prescribed value of 4 NTU. | |||
(vi) The performance characteristics apply to the specification of 1 NTU for water leaving treatment works. | |||
| Aluminium | 10 | 10 | 10 |
| Ammonium | 10 | 10 | 10 |
| Antimony | 25 | 25 | 25 |
| Arsenic | 10 | 10 | 10 |
| Benzene | 25 | 25 | 25 |
| Benzo(a)pyrene | 25 | 25 | 25 |
| Boron | 10 | 10 | 10 |
| Bromate | 25 | 25 | 25 |
| Cadmium | 10 | 10 | 10 |
| Chloride | 10 | 10 | 10 |
| Chromium | 10 | 10 | 10 |
| Colour | 10 | 10 | 10 |
| Conductivity | 10 | 10 | 10 |
| Copper | 10 | 10 | 10 |
| Cyanide f2 | 10 | 10 | 10 |
| 1,2-dichloroethane | 25 | 25 | 10 |
| Fluoride | 10 | 10 | 10 |
| Iron | 10 | 10 | 10 |
| Lead | 10 | 10 | 10 |
| Manganese | 10 | 10 | 10 |
| Mercury | 20 | 10 | 20 |
| Nickel | 10 | 10 | 10 |
| Nitrate | 10 | 10 | 10 |
| Nitrite | 10 | 10 | 10 |
| Pesticides and related products f3 | 25 | 25 | 25 |
| Polycyclic aromatic hydrocarbons f4 | 25 | 25 | 25 |
| Selenium | 10 | 10 | 10 |
| Sodium | 10 | 10 | 10 |
| Sulphate | 10 | 10 | 10 |
| Tetrachloroethene f5 | 25 | 25 | 10 |
| Tetrachloromethane | 20 | 20 | 20 |
| Trichloroethene | 25 | 25 | 10 |
| Trihalomethanes: | 25 | 25 | 10 |
| Total | |||
| Turbidity f6 | 10 | 10 | 10 |
| Turbidity f7 | 25 | 25 | 25 |
Annotations
Footnotes
- f1
*
The composition of m-CP agar is:
Basal medium Tryptose 30.0g Yeast extract 20.0g Sucrose 5.0g L-cysteine hydrochloride 1.0g MgSO4.7H2O 0.1g Bromocresol purple 40.0mg Agar 15.0g Water 1,000.0ml Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine 400.0mg Polymyxine-B sulphate 25.0mg Indoxyl-β-D-glucoside 60.0mg to be dissolved in 8ml sterile water before addition Filter-sterilised 0.5% phenolphthalein diphosphate solution 20.0ml Filter-sterilised 4.5% FeCl3.6H2O 2.0ml - f2
(i)
The method of analysis should determine total cyanide in all forms.
- f3
(ii)
The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.
- f4
(iii)
The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.
- f5
(iv)
The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.
- f6
(v)
The performance characteristics apply to the prescribed value of 4 NTU.
- f7
(vi)
The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.