Division
United Kingdom
F1IF procedure:
- (i)
According to test slide preparation in 4.1(i):
Prepare a set of twofold dilutions of the antibody in IF buffer. The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).
- (ii)
According to test slide preparation in 4.1(ii):
Prepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity.
Figure 1.
Preparation of the test slide according to 4.1(i) and 4.3(i)
| Dilutions of resuspended pellet | |||||||
| 1/100 | 1/1 | 1/1 | 1/1 | 1/1 | | Dilution of resuspended pellet | |
| (T = titre) | T/2 | T/4 | T/2 | T | 2T | | Twofold dilutions of antiserum/antibody |
| Sample 1 |  |  |  |  |  | ||
| 1 | 2 | 3 | 4 | 5 | |||
| Duplicate of sample 1 or sample 2 |  |  |  |  |  | ||
| 6 | 7 | 8 | 9 | 10 | |||
Figure 2.
Preparation of the test slide according to 4.1(ii) and 4.3(ii)
| Working dilution of antiserum/antibody | |||||||
| 1/1 | 1/10 | 1/100 | empty | empty | | Decimal dilution of resuspended pellet | |
| Sample 1 |  |  |  |  |  | ||
| 1 | 2 | 3 | 4 | 5 | |||
| Duplicate of sample 1 or sample 2 |  |  |  |  |  | ||
| 6 | 7 | 8 | 9 | 10 | |||