Appendix F1
United Kingdom
 | 1 Hydrocarbons 2 α-Tocopherol 3 Prenols 4 Triterpenic Alcohols 5 Aliphatic Alcohols 6 Methyl Sterols 7 Sterols 8 Triterpenic Dialcohols |
Figure 1 — TLC of the unsaponifiable fraction from olive pomace oil eluted twice with hexane:diethyl ether (65:35), developed with SO4H2 (50 %) and heated. The bands that should be scrapped are the ones contained within the rectangle, 1 are the bands for aliphatic alcohols and 2 for the sterols and triterpenic dialcohols.
Table I — Relative retention times for sterols
| Peak | Identification | Relative retention time | ||
|---|---|---|---|---|
| SE 54 column | SE 52 column | |||
| 1 | Cholesterol | Δ-5-cholesten-3β-ol | 0,67 | 0,63 |
| 2 | Cholestanol | 5α-cholestan-3β -ol | 0,68 | 0,64 |
| 3 | Brassicasterol | [24S]-24-methyl-Δ-5,22-cholestadien-3β -ol | 0,73 | 0,71 |
| * | Ergosterol | [24S]-24-methyl-Δ-5,7,22 cholestatrien-3β -ol | 0,78 | 0,76 |
| 4 | 24-methylene-cholesterol | 24-methylene-Δ-5,24-cholestadien-3β -o1 | 0,82 | 0,80 |
| 5 | Campesterol | (24R)-24-methyl-Δ-5-cholesten-3β -ol | 0,83 | 0,81 |
| 6 | Campestanol | (24R)-24-methyl-cholestan-3β -ol | 0,85 | 0,82 |
| 7 | Stigmasterol | (24S)-24-ethyl-Δ-5,22-cholestadien-3β -ol | 0,88 | 0,87 |
| 8 | Δ-7-campesterol | (24R)-24-methyl-Δ-7-cholesten-3β -ol | 0,93 | 0,92 |
| 9 | Δ-5,23-stigmastadienol | (24R,S)-24-ethyl-Δ-5,23-choIestadien-3β -ol | 0,95 | 0,95 |
| 10 | Clerosterol | (24S)-24-ethyl-Δ-5,25-cholestadien-3β -ol | 0,96 | 0,96 |
| 11 | ß-sitosterol | (24R)-24-ethyl-Δ-5-cholesten-3β -ol | 1,00 | 1,00 |
| 12 | Sitostanol | 24-ethyl-cholestan-3β -ol | 1,02 | 1,02 |
| 13 | Δ-5-avenasterol | (24Z)-24-ethylidene-Δ-cholesten-3β -ol | 1,03 | 1,03 |
| 14 | Δ-5,24-stigmastadienol | (24R,S)-24-ethyl-Δ-5,24-cholestadien-3β -ol | 1,08 | 1,08 |
| 15 | Δ-7-stigmastenol | (24R,S)-24-ethyl-Δ-7-cholesten-3β -ol | 1,12 | 1,12 |
| 16 | Δ-7-avenasterol | (24Z)-24-ethylidene-Δ-7-cholesten-3β -ol | 1,16 | 1,16 |
| 17 | Erythrodiol | 5α-olean-12-en-3β,28-diol | 1,41 | 1,41 |
| 18 | Uvaol | Δ12-ursen-3β,28-diol | 1,52 | 1,52 |
Figure 2 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from refined olive oil. (1) Cholesterol, (2) α-cholestanol (I.S.), (3) 24-methylencholesterol, (4) campesterol, (5) campestanol, (6) stigmasterol, (7) Δ5,23-stigmastadienol, (8) clerosterol, (9) β-sitosterol, (10) sitostanol, (11) Δ5-avenasterol, (12) Δ5,24-stigmastadienol, (13) Δ7-stigmastenol, (14) Δ7-avenasterol, (15) erythrodiol, (16) uvaol.
Figure 3 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from a lampante olive oil. (1) Cholesterol, (2) α-cholestanol, (3) brassicasterol, (4) 24-methylencholesterol, (5) campesterol, (6) campestanol, (7) stigmasterol, (8) Δ7-campesterol, (9) Δ5,23-stigmastadienol, (10) clerosterol, (11) β-sitosterol, (12) sitostanol, (13) Δ5-avenasterol, (14) Δ5,24-stigmastadienol, (15) Δ7-stigmastenol, (16) Δ7-avenasterol, (17) erythrodiol, (18) uvaol.
Figure 4 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.
Figure 5 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of a refined olive oil and a second centrifugation olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.
Figure 6 — HPLC Chromatogram of an olive oil unsaponifiable separated by HPLC using a UV detector. (1) Aliphatic and triperpenic alcohols; (2) Sterols and triterpenic dialcohols