Division 7.2 DeterminationF1

Once the chromatography conditions have stabilised,

• inject 20 μl of the reference solution C (4.7.1),

• inject 20 μl of the sample solution,

• compare the two chromatograms. Identify the glycyrrhizic acid peaks from their retention times. Measure their areas (or heights) and calculate the concentration in g/l to two decimal figures using the following equation:

  

  where:

  c

• is the concentration in grams per litre of glycyrrhizic acid in the spirit being analysed

C

• is the concentration in grams per litre of ammonium glycyrrhizinate in the reference solution

h

• is the area (or height) of the glycyrrhizic acid peak of the spirit being analysed

H

• is the area (or height) of the glycyrrhizic acid peak of the reference solution

P

• is the purity of the reference ammonium glycyrrhizinate (in %)

823

• is the mass of one mole of glycyrrhizic acid

840

• is the mass of one mole of ammonium glycyrrhizinate.