Division 5.4 HPLC determination
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The following conditions for isocratic elution are offered for guidance; other conditions may be used, provided they yield equivalent results (see also observations 9.1 and 9.2):
| Liquid chromatographic column (4.2): | 125 mm x 4 mm, C18, 3 μm packing or equivalent |
| Column temperature: | Room temperature |
| Mobile phase (3.22): | 3,0 g acetic acid (3.18) + 900 ml water (3.1) +50,0 ml solution (3.21) of 1,1,1-trichloro-2- methyl-2-propanol (3.19) in methanol (3.8) (1 g/100 ml). Adjust pH to 5,0 using ethanolamine (3.20). Make up to 1 000 ml with water (3.1) |
| Flow rate: | 1 ml/min. |
| Total run time: | approx. 34 min. |
| Detection wavelength: | excitation: 280 nm, emission: 356 nm. |
| Injection volume | 20 μl |