Division 6 Calculation of results

United Kingdom

The amount of tryptophane (X), in g per 100g sample, is calculated as follows:

X = \frac{A \times B \times V_{1} \times c \times V_{2} \times M}{C \times D \times V_{3} \times 10 000 \times m}

peak area of internal standard, calibration standard solution (3.17)

peak area of tryptophan, extract (5.2) or hydrolysate (5.3)

V₁

volume in ml (2 ml) of concentrated tryptophan solution (3.15) added to the calibration solution (3.17)

concentration in μmol/ml (= 2,5) of concentrated tryptophan solution (3.15) added to calibration solution (3.17)

V₂

volume in ml of concentrated internal standard solution (3.16) added at the extraction (5.2) (= 5,0 ml) or to the hydrolysate (5.3) (= 2,0 ml)

peak area of internal standard, extract (5.2) or hydrolysate (5.3)

peak area of tryptophan, calibration standard solution (3.17)

V₃

volume in ml (= 2,0 ml) of concentrated internal standard solution (3.16) added to calibration standard solution (3.17)

sample weight in g (corrected to original weight if dried and/or defatted)

molar weight of tryptophan (= 204,23 g/mol)